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. Author manuscript; available in PMC: 2020 Jul 21.
Published in final edited form as: Cell Rep. 2020 Jun 30;31(13):107823. doi: 10.1016/j.celrep.2020.107823

Figure 5. NP Peak as well as Non-peak Regions Participate in RNA-RNA Interactions.

Figure 5.

(A–C) Quantity of RNA-RNA interactions occuring in peak and non-peak regions is shown for wild-type WSN (A), the hotspot mutant (B), and wild-type WSN

derived from SPLASH (C). The bottom and top of the boxes represent the 25th and 75th percentiles; the bar in the middle represents the median value, and the whiskers represent 1.53 the interquartile range extended out from the 25th and 75th percentiles. The values on the y axis represent the absolute number of mapped RNA hybrids at each nucleotide, which were plotted based on their classification of overlapping with either a called NP peak or a non-peak (Lee et al., 2017); 3,958 and 9,623 nucleotides of the WSN IAV genome were called as peaks and non-peaks, respectively. Please note that values are dependent on sequencing coverage (SPLASH data contained more sequencing reads than the 2CIMPL data, hence the higher values); p values were determined using the Wilcoxon signed-rank test. See also Figures S5.