Fig. 1.

Freeze-drying formulations #2 and #3 effectively protected the activity of poxvirus vaccine. The infectivity of recombinant poxvirus vaccines (rTTV-OVA) protected by six formulations were detected post lyophilization and in different storage conditions. The titer is detected by counting the blue viral plaques per well. A Titers of poxvirus in six formulations post lyophilization. The titer of FR3 was slightly higher than that of the other formulations (**P < 0.01). B–C The protected rTTV-OVAs were stored at 4 °C (B) or 25 °C (C) for 7/14/28 days post lyophilization. The titers of vaccines in FR2 and FR3 are higher than that in any other formulation.