Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 20;20:233. doi: 10.1186/s12876-020-01383-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — The flowchart of this study. First, Melatonin inhibited cell growth of HGC-27 and HCT-8 cells in a dose and time-dependent manner. The concentration (2.5 mmol/L) and time (24 h) of melatonin for treatment were determined. Second, four cancer cell lines (HGC-27, HCT-8, Huh-7 and HepG2) across three types of gastrointestinal carcinomas (GC, CRC and HCC) were treated by melatonin for 24 h and performed by DNA microarray analysis. Third, the DEGs by melatonin treatment detected by Student’s t-test and the reproducibility-based PD were combined to investigate the common biological signaling pathways altered by melatonin. Fourth, the DEGs detected between tumor and normal tissues but reversed by melatonin in cancer cell lines were used to explore the potential anticancer effects of melatonin. Finally, the 5-FU resistance-related genes in GC and CRC but reversed by melatonin in cancer cell lines were used to explore the potential of melatonin to increase the sensitivity of 5-FU in GC and CRC