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. 2020 Jul 20;10:88. doi: 10.1186/s13578-020-00450-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Macrophage-derived supernatants inhibit adipocyte differentiation of hADSCs. a Scheme of the experimental procedure. hADSCs (2 × 10⁴) were plated and cultured with adipogenesis-inducing medium (AD) with or without the supernatants from different macrophage subtypes at the ratio of 1:1 in 48-well plate, medium was changed every 3 days. b hADSCs cells that were induced to undergo adipogenic differentiation were fixed for triglycerides staining with Oil Red O to show lipid droplets. c Western blot analysis of the protein levels of PPAR-γ in hADSCs cultured in different conditions for 5 days. d Expression of adipogenic genes were measured by Q-PCR assay on day 3. e hADSCs were pre-treated with the specific supernatant for 24 h, then changed to adipogenic inducing medium without supernatant, lipid droplets in hADSCs were revealed by Oil Red O staining after 9 days. Scale bars are 100 μm