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Designed primer sequences with the respective annealing temperature and amplicon size for gene amplification
| Target gene | Primer name | Sequence (5ʹ-3ʹ) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
| 12S rRNA | 12S.C1.F | GTGCCAGCTAYCGCGGTTA | 55 | 460 |
| 12S.C1.R | GRTGACGGGCRATATGTG | |||
| 12S.C345.F | GTWCCAGAATAATCGGMTA | 46 | ||
| 12S.C345.R | ATTGAYGGATGRTTTGTRC | |||
| 16S rRNA | 16S.C1.F | ACGAGAAGACCCTRGRAAYT | 50 | 240 |
| 16S.C1.R | GRTYTAAACTCAAATCACG | |||
| 16S.C345.F | AAGATAAGTCTTYGGAARYT | 45 | ||
| 16S.C345.R | GAAYTAAACTAATATCAM G |
