Table 2.
Overview of results obtained during the determination of the optimal threshold for MALDI-TOF MS identification
| Species in the database | Optimal threshold | TP | FN | TN | FP | Sensitivity | Specificity | PPV | NPV | AUC | 95% CI (AUC) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| An. bancroftii | 1.48 | 7 | 0 | 217 | 0 | 1 | 1 | 1 | 1 | 1 | 1–1 |
| Ae. scutellaris | 1.75 | 9 | 1 | 214 | 0 | 0.9 | 1 | 0.9 | 1 | 0.90 | 0.77–1 |
| Ae. aegypti | 1.77 | 18 | 1 | 205 | 0 | 0.97 | 1 | 0.95 | 1 | 0.97 | 0.92–1 |
| Ae. notoscriptus | 1.75 | 19 | 0 | 205 | 0 | 1 | 1 | 1 | 1 | 1 | 1–1 |
| Ae. vigilax | 1.57 | 17 | 3 | 204 | 0 | 0.85 | 1 | 0.85 | 1 | 0.85 | 0.75–0.96 |
| Ae. vexans | 1.57 | 16 | 4 | 204 | 0 | 0.8 | 1 | 0.80 | 1 | 0.85 | 0.75–0.96 |
| Cx. quinquefasciatus | 1.79 | 29 | 1 | 190 | 4 | 0.97 | 0.98 | 0.97 | 0.98 | 0.97 | 0.92–1 |
| Cx. iyengari | 1.77 | 18 | 0 | 205 | 1 | 1 | 0.99 | 1 | 0.99 | 0.99 | 0.99–1 |
| Cx. sitiens | 1.64 | 20 | 0 | 214 | 0 | 1 | 1 | 1 | 1 | 1 | 1–1 |
| Cx. annulirostris | 1.78 | 29 | 1 | 190 | 4 | 0.97 | 0.98 | 0.97 | 0.98 | 0.99 | 0.97–1 |
| T. melanesiensis | 1.7 | 20 | 0 | 204 | 0 | 1 | 1 | 1 | 1 | 1 | 1–1 |
| Six speciesa | 1.70 | 109 | 8 | 100 | 7 | 0.93 | 0.93 | 0.93 | 0.93 | 0.94 | 0.91–0.97 |
| Five speciesb | 1.63 | 101 | 6 | 110 | 7 | 0.94 | 0.95 | 0.94 | 0.94 | 0.96 | 0.94–0.99 |
aAn. bancroftii, Ae. scutellaris, Ae. aegypti, Ae. vigilax, Cx. quinquefasciatus, Cx. sitiens
bAe. notoscriptus, Ae. vexans, Cx. iyengari, Cx. annulirostris, T. melanesiensis
Abbreviations: AUC, area under the curve; TP, true positives; FN, false negatives; TN, true negatives; FP, false positives; PPV, positive predictive values; NPV, negative predictive values