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. 2020 Jul 21;13:131. doi: 10.1186/s13068-020-01767-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Development of a β-galactosidase reporter system and selection of strong promoters in Schizochytrium sp. a Construction of reporter plasmid pPICZαA-lacZ. b PCR verification of transformants. +: positive control, reporter plasmid was used as template; −: negative control, WT genomic DNA was used as template; AOX1p-lacZ, ubip-lacZ, EF-1αp-lacZ, ccg1p-lacZ, and TEF-1p-lacZ: the transformants of pPICZαA-AOX1p-lacZ, pPICZαA-ubiquitinp-lacZ, pPICZαA-EF-1αp-lacZ, pPICZαA-ccg1p-lacZ, and pPICZαA-TEF-1p-lacZ. c Phenotypes of the corresponding transformants. Cells were grown for 96 h on GPY plates with 40 μg/mL X-gal. d β-galactosidase enzymatic activities of the transformants. Cells were grown in seed medium for 64 h