Fig. 5.

Effect of ACL and ACC overexpression on lipid accumulation and DHA production by Schizochytrium sp. a Dry cell weight (DCW, g/L). b Total lipids (% DCW). c DHA yield (g/L). Cells were cultured in fermentation medium for 120 h. d Imaging analyses of WT, OACL, OACC, and OACL-ACC. The 48-h cultured cells were stained with Nile red dye for neutral lipid staining. Scale bar, 10 µm. Error bars: SD from three independent experiments. Data were analyzed by one-way ANOVAs and Duncan’s multiple range tests in SPSS version 23.0. In a–c, columns with different lowercase letters are significantly different at P < 0.05