Table 2.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Schizochytrium sp. | ||
| ATCC 20888 | Wild-type strain (WT) | American Type Culture Collection |
| AOX1p-lacZ | WT strain carrying pPICZαA-AOX1p-lacZ | This study |
| ubip-lacZ | WT strain carrying pPICZαA-ubiquitinp-lacZ | This study |
| TEF-1p-lacZ | WT strain carrying pPICZαA-TEF-1p –lacZ | This study |
| EF-1αp-lacZ | WT strain carrying pPICZαA-EF-1αp-lacZ | This study |
| ccg1p-lacZ | WT strain carrying pPICZαA-ccg1p-lacZ | This study |
| OACL | ACL overexpression strain | This study |
| OACC | ACC overexpression strain | This study |
| OACL-ACC | ACL and ACC co-overexpression strain | This study |
| E. coli | ||
| JM109 | General cloning host for plasmid manipulation | Laboratory stock |
| Plasmids | ||
| pPICZαA | Yeast expression vector | [42] |
| pPICZαA-AOX1p-lacZ | lacZ reporter vector using AOX1 promoter and terminator | This study |
| pPICZαA-ubiquitinp-lacZ | lacZ reporter vector using ubiquitin promoter and terminator | This study |
| pPICZαA-TEF-1p-lacZ | lacZ reporter vector using TEF-1 promoter and terminator | This study |
| pPICZαA-EF-1αp-lacZ | lacZ reporter vector using EF-1α promoter and terminator | This study |
| pPICZαA-ccg1p-lacZ | lacZ reporter vector using ccg1 promoter and terminator | This study |
| pPICZαA-ACL | ACL overexpression vector based on pPICZαA | This study |
| pPICZαA-ACC | ACC overexpression vector based on pPICZαA | This study |
| pPICZαA-ACL-ACC | ACL and ACC co-overexpression vector based on pPICZαA | This study |