Fig. 1.

Morphology-biased serial transfer for generation of salt resilient Z. mobilis strains. The picture shows the fully grown Zm6 culture in the complex medium supplemented with NaCl 0.225 M. The sediments of Zm6 Z. mobilis cells at the bottom of culture (pointed by a white arrowhead), constituting clumps of bulged/filamented cells, were observed under a phase contrast microscope. In the upper layer of the medium (pointed by a gray arrowhead), shorter filamented cells resided, and this part of culture was transferred as an inoculum for next round of evolution