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. 2020 Jul 20;19:147. doi: 10.1186/s12934-020-01406-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Cell sizes are reduced in the evolved strains under non-saline conditions. Histograms (top two and left bottom panel) show the distribution of cellular length of growing Zm6 (top left), KFS1 (top right) and KFS2 (left bottom) under the non-saline condition (N = 150 for Zm6, N = 133 for KFS1 and KFS2). The cells were grown in the complex medium under anaerobic condition until OD₆₀₀ of the cultures reached around 1 in all strain. Cells were then mounted on the agarose-pad and imaged by a phase contrast microscope. Kernel density was plotted using bandwidth of 0.289 for all histograms. Phase contrast images of each strain under non-saline condition (left bottom, strain is indicated in the image) is shown with a scale bar 10 μm applied for all images. Small table in the right bottom displays the mean value of cellular length (μm) in each strain. SD stands for standard deviation