Table 1.
Genomic alterations identified by NGS in each tumor component
| Genomic Alteration | Variant Allele Fraction or % mosaicism | ||||
|---|---|---|---|---|---|
| Gene | DNA Change | Protein Change | Oligodendroglioma | Astrocytoma | Initial Resection |
| IDH1 | c.395G > A (NM_005896.3) | p.Arg132His | 0.31 | 0.29 | 0.43 |
| TERT | c.-124C > T (NM_198253.2) | p.? | 0.23 | 0 | 0 |
| CIC | c.781_792del (NM_015125.4) | p.Phe261_His264del | 0.32 | 0 | 0 |
| – | 1p/19q co-deletion | – | 35–45% | not detected (< 30%) | not detected (< 30%) |
| TP53 | c.841G > A (NM_000546.5) | p.Asp281Asn | < 0.01 | 0.38 | 0.87 |
| TP53 | c.817C > T (NM_000546.5) | p.Arg273Cys | < 0.01 | 0.08 | 0.08 |
| ATRX | c.4744dup (NM_000489.4) | p.Thr1582Asnfs*19 | < 0.01 | 0.26 | 0.26 |
| NOTCH1 | c.1347C > G (NM_017617.4) | p.Cys449Trp | 0 | 0.18 | 0.18 |
| PIK3CA | c.3197C > T (NM_006218.3) | p.Ala1066Val | 0 | 0 | 0.05 |
| MDM4 | c.458A > G (NM_002393.4) | p.Asp153Gly | 0.39 | 0.44 | 0.44 |