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. 2020 Jul 20;20:328. doi: 10.1186/s12935-020-01418-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — EO771 cells display a luminal B phenotype. a–d The relative expression of mRNA coding for ERα (a), ERβ (b), PR (c) and ERBB2 (d) was evaluated on MCF-7, MDA-MB-231 and EO771 cells. The values are normalized to the GAPDH gene expression. The data from MCF-7 were set to 1 and the relative quantity of mRNA is shown. P values of < 0.05 (*) using a Wilcoxon-Mann Whitney test indicate a significant difference. E–H: ERα (e), ERβ (f), PR (g) and ERBB2 (h) protein levels was assayed by western blot on MCF-7, MDA-MB-231 and EO771 cells (representative of 3 experiments) and normalized to the GAPDH or actin protein levels