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. 2020 Jul 20;20:678. doi: 10.1186/s12885-020-07180-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Detection results of target cells overexpressing PDL1 and(or) MUC16. a Detection results of PDL1 antigen on OVCAR3-PDL1-luc cells. OVCAR3-PDL1-luc cells stained with 70ul Steady-Glo for 20 min in the dark. b Detection results of MUC16 antigen on OVCAR3-MUC16-GFP-luc cells. GFP is used to mark the MUC16. c Detection results of MUC16 and PDL1 antigens on OVCAR3-MUC16-GFP-PDL1-luc cells. OVCAR3-MUC16-GFP-PDL1-luc cells stained with 70ul Steady-Glo as the above methods. Control groups are the green peak; Experimental groups are the red peak. The top pictures show the titer test results, and the bottom two show the cell pools and the monoclonal samples