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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Kidney Int. 2019 Sep 5;97(2):340–349. doi: 10.1016/j.kint.2019.08.022

Figure 3. Effect of CpG-ODN and IL-6 on cultured splenocytes of ddY mice.

Figure 3.

(A) Serum levels of IL-6 in CpG-ODN-injected mice were significantly higher compared with control mice. *P<0.05. CpG-ODN stimulation increased production of IL-6 of splenocytes of ddY mice in culture. Incubation of splenocytes of ddY mice with recombinant IL-6 increased production of total IgA and aberrantly glycosylated IgA, resulting in the formation of IgG-IgA IC. Recombinant IL-6 increased overproduction of APRIL. Bars represent the mean±SEM. **P<0.01. (B) Splenocytes in culture were stimulated with CpG-ODN in the presence or absence of IL-6-neutralizing antibody. Anti-IL-6 antibody reduced production of total IgA and aberrantly glycosylated IgA, and, consequently, reduced formation of IgG-IgA IC induced by CpG-ODN. **P<0.01. PBS, Phosphate-buffered saline.