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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Kidney Int. 2019 Sep 5;97(2):340–349. doi: 10.1016/j.kint.2019.08.022

Figure 4. Effect of CpG-ODN or APRIL supplementation on human IgA1-secreting cell lines on IgA1, Gd-IgA1, and IL-6 production.

Figure 4.

(A) CpG-ODN supplementation enhanced production of IL-6. CpG-ODN enhanced gene expression of APRIL. Western-blot analysis demonstrated that CpG-ODN increased protein levels of APRIL. Results were evaluated densitometrically. Stimulation with CpG-ODN induced production of IgA and Gd-IgA1. Bars represent the mean±SEM. *P<0.05, **P<0.01. (B) APRIL siRNA knock-down reduced IL-6-induced overproduction of IgA and Gd-IgA1. *P<0.05, **P<0.01. (C) IgA1-producing cells stimulated with recombinant APRIL produced more IgA and Gd-IgA1. (D) IgA1-secreting cells were incubated with anti-IL-6 antibody after CpG-ODN stimulation. The enhanced production of IgA and Gd-IgA1 induced by CpG-ODN was reduced by anti-IL-6 antibody and by siRNA knock-down of APRIL. PBS, Phosphate Buffered Saline.