Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 14;14:646. doi: 10.3389/fnins.2020.00646

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Ma, Fu, Cao, Shao, Song, Sheng, Yang, Cui, Chen, Zhao, Luo, Lai and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Neural circuit mediated by the POR from the CA1 of dorsal hippocampus to the BLA in saline-treated mice and mice with conditioned place aversion. (A) Left panel: diagram of the injection sites of viruses in the CA1 of dorsal hippocampus and the POR. Right panel: the CPA scores of saline + saline group and morphine + naloxone group (n = 4 in saline + saline group, n = 5 in morphine + naloxone group, *p = 0.004, compared with pre-test, two-way ANOVA, Bonferroni post hoc analysis). (B) Left top panel: the expression of Cre-EGFP (green-colored) in the CA1 of dorsal hippocampus after post-test in saline + saline group. Scale bar = 500 μm. The magnified image of the CA1 of dorsal hippocampus is shown on the top. Scale bar = 100 μm. Right top panel: Cre positive neurons (green-colored) and the expression of tdTomato-WPRE (red-colored) in the POR after post-test in saline + saline group. Scale bar = 500 μm. The magnified image of the POR is shown on the bottom. Scale bar = 100 μm. Down panel: the tdTomato positive fibers (red-colored) in the BLA in saline + saline group. Scale bar = 100 μm. (C) Left top panel: the expression of Cre-EGFP (green-colored) in the CA1 of dorsal hippocampus after post-test in morphine + naloxone group. Scale bar = 500 μm. The magnified image of the CA1 of dorsal hippocampus is shown on the top. Scale bar = 100 μm. Right top panel: Cre positive neurons (green-colored) and the expression of tdTomato-WPRE (red-colored) in the POR after post-test in morphine + naloxone group. Scale bar = 500 μm. The magnified image of the POR is shown on the bottom. Scale bar = 100 μm. Down panel: the tdTomato positive fibers (red-colored) in the BLA in morphine + naloxone group. Scale bar = 100 μm. (D) The density of Cre positive neurons of the POR in each group (n = 3 in saline + saline group, n = 2 in morphine + naloxone group, *p = 0.0360, compared to saline + saline group, student’s t-test). (E) The density of tdTomato positive fibers in the BLA in each group (n = 3 in each group, *p = 0.0352, compared to saline + saline group, student’s t-test). Data are shown as the mean ± SEM.