(A) Anti-GRP94 antibody captured cargo from SKBr3 cell extracts, and the remaining supernatant was separated under native and denaturing conditions and immunoblotted with the indicated antibodies.+, 1 times antibody amount; ++, 2 times antibody amount. IgG, control; red arrow, unspecific signal.
(B) Immuno-capture as in (A) of SKBr3 cells treated with PU-WS13 in a dose and time-dependent manner. Graph, mean of 3 independent experiments; error bars, SEM.
(C) Summary of the findings.