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. Author manuscript; available in PMC: 2020 Jul 21.
Published in final edited form as: Cell Rep. 2020 Jun 30;31(13):107840. doi: 10.1016/j.celrep.2020.107840

Figure 6. Accumulation of hgGRP94 at the PM Is Sufficient to Augment HMW GRP94 Levels, Stabilize Receptors at the PM, and RewireProtein Networks in the Cytosol.

Figure 6.

(A) Biochemical profile of GRP94 in the indicated cell fractions obtained from cells containing the WT (1) or the TM96 (2) GRP94 construct. LRP6, control for PM proteins; p-p65, p-ERK control for signaling activity and HSP90-incorporating epichaperomes for oncogenic activation of cytosolic chaperomes; myc, control for the presence of TM96. Gels are representative of three individual experiments.

(B) Western blot of indicated cells treated for 24 h with PU-WS13 (10 μM). Graphs, mean ± SEM; n = 3; one-way ANOVA with Dunnett’s post hoc, ***p < 0.001.

(C) Anchorage-independent growth in soft agar examined for MethA WT and TM96 cells at day 21. Graph, mean ± SEM; n = 3 experiments; unpaired t test, **p < 0.01.

(D) Viability of cells treated with PU-WS13 for 72 h. Graph, mean ± SEM; n = 4; two-way ANOVA, ****p < 0.0001. Negative y axis values depict killing of the initial cell population.

(E) Schematic of the findings.

See also Figure S6.