KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-HSP90 | StressMarq Biosciences | Cat# SMC-107, RRID:AB_854214 |
| Mouse monoclonal anti-Calnexin | BD Biosciences | Cat# 610523, RRID:AB_397883 |
| Rabbit polyclonal anti-HER2 | Invitrogen | Cat# 28–0004 |
| Mouse monoclonal anti-HSP70 | Enzo Life Sciences | Cat# ADI-SPA-810, RRID:AB_10615203 |
| Rat monoclonal anti-GRP94 | Enzo Life Sciences | Cat# ADI-SPA-850, RRID:AB_11179746 |
| Mouse monoclonal anti-GAPDH | Abcam | Cat# ab8245, RRID:AB_2107448 |
| Rabbit polyclonal anti-GRP78 | Abcam | Cat# ab21685, RRID:AB_2119834 |
| Rabbit polyclonal anti-HSP90α | Abcam | Cat# ab2928, RRID:AB_303423 |
| Mouse monoclonal anti-EEA1 | Abcam | Cat# ab70521, RRID:AB_1603734 |
| Rabbit polyclonal anti-Calnexin | Abcam | Cat# ab22595, RRID:AB_2069006 |
| Rabbit polyclonal anti-Cleaved PARP | Promega | Cat# G7341, RRID:AB_430876 |
| Rabbit monoclonal anti-EGFR | Cell Signaling Technology | Cat# 4267, RRID:AB_2246311 |
| Rabbit monoclonal anti-LRP6 | Cell Signaling Technology | Cat# 2560, RRID:AB_2139329 |
| Rabbit polyclonal anit-p-AKT (S473) | Cell Signaling Technology | Cat# 9271, RRID:AB_329825 |
| Rabbit monoclonal anti-AKT | Cell Signaling Technology | Cat# 4691, RRID:AB_915783 |
| Mouse monoclonal anti-Caspase 7 | Cell Signaling Technology | Cat# 9494, RRID:AB_2068141 |
| Rabbit monoclonal anti-p-ERK1/2 (T202/Y204) | Cell Signaling Technology | Cat# 4377, RRID:AB_331775 |
| Rabbit monoclonal anti-ERK1/2 | Cell Signaling Technology | Cat# 4695, RRID:AB_390779 |
| Rabbit monoclonal anti-p-STAT3 (Y705) | Cell Signaling Technology | Cat# 9145, RRID:AB_2491009 |
| Rabbit monoclonal anti-STAT3 | Cell Signaling Technology | Cat# 12640, RRID:AB_2629499 |
| Rabbit polyclonal anti-Flotillin-1 | Cell Signaling Technology | Cat# 3253, RRID:AB_2106734 |
| Rabbit monoclonal anti-p-p65 (S536) | Cell Signaling Technology | Cat# 3033, RRID:AB_331284 |
| Rabbit monoclonal anti-p65 | Cell Signaling Technology | Cat# 8242, RRID:AB_10859369 |
| Rabbit polyclonal anti-GRP94 | Sigma-Aldrich | Cat# G4420, RRID:AB_477017 |
| Mouse monoclonal anti-α-Tubulin | Sigma-Aldrich | Cat# T5168, RRID:AB_477579 |
| Mouse monoclonal anti-β-actin | Sigma-Aldrich | Cat# A1978, RRID:AB_476692 |
| Concanavalin A-HRP | Sigma-Aldrich | Cat# L6397 |
| anti-HER2-FITC | BD Biosciences | Cat# 340553, RRID:AB_2099395 |
| Annexin V-FITC | BD Biosciences | Cat# 556419, RRID:AB_2665412 |
| Goat anti-rat IgG-HRP | Santa Cruz Biotechnology | Cat# sc-2006, RRID:AB_1125219 |
| Goat anti-Rabbit Ig-HRP | SouthernBiotech | Cat# 4010–05, RRID:AB_2632593 |
| Sheep Anti-Mouse IgG-HRP | GE Healthcare | Cat# NXA931, RRID:AB_772209 |
| Mouse monoclonal anti-Myc | Thermo Fisher Scientific | Cat# R950–25, RRID:AB_2556560 |
| Goat anti-Rabbit IgG-Cy3 | Thermo Fisher Scientific | Cat# A10520, RRID:AB_2534029 |
| Goat anti-Rabbit IgG-Cy5 | Thermo Fisher Scientific | Cat# A10523, RRID:AB_2534032 |
| Goat anti-Mouse IgG-Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A28175, RRID:AB_2536161 |
| Bacterial | ||
| Stbl3 Chemically Competent E. coli | Thermo Fisher Scientific | Cat#C737303 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| PU-WS13 | Chiosis Lab | N/A |
| PU-29F | Chiosis Lab | N/A |
| PU-H71 | Chiosis Lab | N/A |
| HJP-149 | Chiosis Lab | N/A |
| SO-33 | Chiosis Lab | N/A |
| PU-WS13-biotin | Chiosis Lab | N/A |
| Inactive-WS13-biotin | Chiosis Lab | N/A |
| Paclitaxel | Selleck Chemicals | Cat#S1150 |
| Erlotinib | Selleck Chemicals | Cat# S1023 |
| Lapatinib | Selleck Chemicals | Cat# S2111 |
| Cetuximab | Leftover from the MSKCC pharmacies | N/A |
| Critical Commercial Assays | ||
| Minute Plasma Membrane Protein Isolation and Cell Fractionation Kit | Invent Biotechnologies | Cat# SM005 |
| ProteoExtract® Subcellular Proteome Extraction Kit | Millipore Sigma | Cat# 539790 |
| CellTiter-Glo® 2.0 Cell Viability Assay | Promega | Cat# G9242 |
| T7E1 assay | NEB | Cat# M0302 |
| De-glycosylation by Endo H | NEB | Cat# P0703 |
| De-glycosylation by PNGase F | NEB | Cat# P0709 |
| Lambda Protein Phosphatase | NEB | Cat# P0753 |
| Phusion Flash High-Fidelity PCR Master Mix | Thermo Fisher Scientific | Cat # F548 |
| Neon Transfection System 100 μL Kit | Thermo Fisher Scientific | Cat# MPK10025 |
| Pierce Cell Surface Protein Isolation Kit | Thermo Fisher Scientific | Cat# 89881 |
| Lipofectamine RNAiMAX Transfection Reagent | Thermo Fisher Scientific | Cat# 13778075 |
| Deposited Data | ||
| Proteomic Data | This study | MassIVE (MSV000085459); ftp://massive.ucsd.edu/MSV000085459/ |
| Experimental Models: Cell Lines | ||
| Human: HMEC | ATCC | Cat# PCS-600–010 |
| Human: SKBr3 | ATCC | Cat# HTB-30, RRID:CVCL_0033 |
| Human: MDA-MB-468 | ATCC | Cat# HTB-132, RRID:CVCL_0419 |
| Human: HCC1806 | ATCC | Cat# CRL-2335, RRID:CVCL_1258 |
| Human: MDA-MB-231 | ATCC | Cat# HTB-26, RRID:CVCL_0062 |
| Human: MDA-MB-415 | ATCC | Cat# HTB-128, RRID:CVCL_0621 |
| Human: MCF-7 | ATCC | Cat# HTB-22, RRID:CVCL_0031 |
| Human: BT-474 | ATCC | Cat# HTB-20, RRID:CVCL_0179 |
| Human: BT-20 | ATCC | Cat# HTB-19, RRID:CVCL_0178 |
| Human: MDA-MB-361 | ATCC | Cat# HTB-27, RRID:CVCL_0620 |
| Human: MDA-MB-453 | ATCC | Cat# HTB-131, RRID:CVCL_0418 |
| Human: T47D | ATCC | Cat# HTB-133, RRID:CVCL_0553 |
| Human: AU565 | ATCC | Cat# CRL-2351, RRID:CVCL_1074 |
| Mouse: MethA WT | Dr. Z. Li, OSU | N/A |
| Mouse: MethA TM96 | Dr. Z. Li, OSU | N/A |
| Human: MiaPaCa2 | ATCC | Cat# CRL-1420, RRID:CVCL_0428 |
| Human: Panc1 | ATCC | Cat# CRL-1469, RRID:CVCL_0480 |
| Human: BxPC3 | ATCC | Cat# CRL-1687, RRID:CVCL_0186 |
| Human: CAPAN1 | ATCC | Cat# HTB-79, RRID:CVCL_0237 |
| Human: SU86.86 | ATCC | Cat# CRL-1837, RRID:CVCL_3881 |
| Human: HPAF2 | ATCC | Cat# CRL-1997, RRID:CVCL_0313 |
| Human: 931019 | Dr. Y. Janjigian, MSKCC | N/A |
| Human: 931102 | Dr. Y. Janjigian, MSKCC | N/A |
| Human: ASPC1 | ATCC | Cat# CRL-1682, RRID:CVCL_0152 |
| Human: CAPAN2 | ATCC | Cat# HTB-80, RRID:CVCL_0026 |
| Human: PL45 | ATCC | Cat# CRL-2558, RRID:CVCL_3567 |
| Human: CFPAC | ATCC | Cat# CRL-1918, RRID:CVCL_1119 |
| Human: NCI-H3122 | Dr. M. Moore, MSKCC | RRID:CVCL_5160 |
| Human: NCI-H2228 | Dr. M. Moore, MSKCC | RRID:CVCL_1543 |
| Human: NCI-H1373 | Dr. N. Lecomte, MSKCC | RRID:CVCL_1465 |
| Human: NCI-H525 | Dr. N. Lecomte, MSKCC | N/A |
| Human: NCI-N87 | ATCC | Cat# CRL-5822, RRID:CVCL_1603 |
| Human: SNU-1 | ATCC | Cat# CRL-5971, RRID:CVCL_0099 |
| Human: PEO-1 | Dr. D. Solit, MSKCC | RRID:CVCL_Y032 |
| Human: PEO-4 | Dr. D. Solit, MSKCC | RRID:CVCL_2690 |
| Human: OVCAR4 | Dr. D. Solit. MSKCC | RRID:CVCL_1627 |
| Human: OV1847 | Dr. D. Solit. MSKCC | RRID:CVCL_D703 |
| Human: A2780 | Dr. D. Solit, MSKCC | RRID:CVCL_0134 |
| Human: IGROV-1 | Dr. D. Solit, MSKCC | RRID:CVCL_1304 |
| Human: OVCAR5 | Dr. D. Solit, MSKCC | RRID:CVCL_1628 |
| Human: SKRC38 | Dr. S. Larson, MSKCC | RRID:CVCL_6189 |
| Human: SKRC52 | Dr. S. Larson, MSKCC | RRID:CVCL_6198 |
| Human: LAN5 | Children’s Oncology Group (COG) | RRID:CVCL_0389 |
| Human: SMS-KCNR | Children’s Oncology Group (COG) | RRID:CVCL_7134 |
| Human: SY5Y | ATCC | Cat# CRL-2266, RRID:CVCL_0019 |
| Human: TC71 | Dr. S Ambati, MSKCC | RRID:CVCL_2213 |
| Human: A673 | Dr. S Ambati, MSKCC | RRID:CVCL_0080 |
| Human: SU-DHL4 | DSMZ | Cat# ACC-495, RRID:CVCL_0539 |
| Human: SU-DHL6 | ATCC | Cat# CRL-2959, RRID:CVCL_2206 |
| Human: Toledo | ATCC | Cat# CRL-2631, RRID:CVCL_3611 |
| Human: Karpas422 | DSMZ | Cat# ACC-32, RRID:CVCL_1325 |
| Human: OCI-Ly1 | Ontario Cancer Institute | RRID:CVCL_1879 |
| Human: OCI-Ly7 | Ontario Cancer Institute | RRID:CVCL_1881 |
| Human: Farage | ATCC | Cat# CRL-2630, RRID:CVCL_3302 |
| Human: RCK8 | DSMZ | Cat# ACC-561, RRID:CVCL_1883 |
| Human: HBL1 | Dr. J. Angel Martinez-Climent | RRID:CVCL_4213 |
| Human: OCI-Ly3 | Ontario Cancer Institute | RRID:CVCL_8800 |
| Human: U2932 | Dr. J. Angel Martinez-Climent | RRID:CVCL_1896 |
| Human: MD901 | Dr. J. Angel Martinez-Climent | RRID:CVCL_D709 |
| Human: TMD8 | Dr. L. M. Staudt, NIH | RRID:CVCL_A442 |
| Human: BC3 | ATCC | Cat# CRL-2277, RRID:CVCL_1080 |
| Human: Kasumi-1 | ATCC | Cat# CRL-2724, RRID:CVCL_0589 |
| Human: MOLM13 | DSMZ | Cat# ACC-554, RRID:CVCL_2119 |
| Human: K562 | ATCC | Cat# CCL-243, RRID:CVCL_0004 |
| Human: U266 | Dr. Z. Li, OSU | RRID:CVCL_0566 |
| Human: PCNY1 | Dr. Z. Li, OSU | N/A |
| Human: MM.1R | Dr. Z. Li, OSU | Cat# CRL-2975, RRID:CVCL_8794 |
| Experimental Models: Organisms/Strains | ||
| Mouse: Hsd:Athymic Nude-Foxn1nu | ENVIGO RMS, INC. | Outbred, Stock #: 069, RRID:MGI:5652489 |
| Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ | The Jackson Laboratory | IMSR Cat# JAX:005557, RRID:IMSR_JAX:005557 |
| Oligonucleotides | ||
| List of oligos See | STAR Methods | N/A |
| Recombinant DNA | ||
| PX458 | Addgene | RRID:Addgene_48138 |
| Software and Algorithms | ||
| Prism V6 | GraphPad software | https://www.graphpad.com |
| FloJo | FlowJo LLC | https://www.flowjo.com |
| LAS AF LITE | Leica | https://leica-las-af-lite.software.informer.com/ |
| FIJI | IMAGEJ | https://imagej.net/Fiji |
| Proteome Discoverer 1.4 | Thermo Fisher Scientific | https://www.thermofisher.com/order/catalog/product/OPTON-30795 |
| Thermo Scientific Xcalibur | Thermo Fisher Scientific | https://www.thermofisher.com/order/catalog/product/OPTON-30487 |
| Mascot | Matrix Science | http://www.matrixscience.com/ |
| Other | ||
| Protein A Agarose | Roche | Cat# 11134515001 |
| Protein G Agarose | Roche | Cat# 11243233001 |
| Pierce High Capacity Streptavidin Agarose | Thermo Fisher Scientific | Cat# 20361 |
| Pierce ECL Western Blotting Substrate | Thermo Fisher Scientific | Cat# 32109 |
| UltraPure LMP Agarose | Thermo Fisher Scientific | Cat# 16520–100 |
| ProLong Gold Antifade Mountant with DAPI | Thermo Fisher Scientific | Cat# P36935 |
| Lab-Tek II Chamber Slide System | Thermo Fisher Scientific | Cat# 154461 |
| Pierce 16% Formaldehyde (w/v), Methanol-free | Thermo Fisher Scientific | Cat# 28908 |
| RPMI with 1% Penicillin/Streptomycin | MSKCC Media Core | N/A |
| DMEM with 1% Penicillin/Streptomycin | MSKCC Media Core | N/A |
| FBS | VWR | Cat# 97068–085 |
| 7-AAD Viability Staining Solution | Biolegend | Cat# 420404 |
| Corning® Matrigel® Basement Membrane Matrix, Phenol Red-free | Corning | Cat# 356237 |