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. 2020 Jul 14;13:113. doi: 10.3389/fnmol.2020.00113

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Copyright © 2020 Shah, Mittal, Shahal, Sinha, Bulut, Mittal and Eshraghi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TDCA prevents EIT induced loss of mitochondrial membrane potential (MMP). (A) OC explants were stained with JC-1 probe to determine MMP. EIT exposed OCs showed red and green staining indicating loss of MMP whereas explants in control and TDCA treated group showed only red staining suggesting preservation of MMP. (B) The mean red/green staining signal intensity ratio was calculated using ImageJ software. Data are expressed as mean values ± SD and are representative of three independent experiments. *P < 0.001 compared to the EIT group. n = 6 OCs per group. Scale bars: 10 μm.