Figure 1.

Structure-Based Design of Chimeric Lyssavirus Glycoproteins

(A–C) Structural models of lyssavirus glycoprotein (G) ectodomain monomers. (A) Representative structural model of a lyssavirus G with proposed structural domains highlighted (clip in yellow, core in orange, flap in red). Structural model of Chimeric G 1 (B) and Chimeric G 2 (C) clip domains highlighted in white, and core and flap domains highlighted in blue or red, corresponding to patterns in (E) and (F).

(D–F) Linear schematics of a representative lyssavirus G monomer (D) and the RABV G/MOKV G chimeric Gs named Chimeric G 1 (E) and Chimeric G 2 (F). In (D), proposed structural domains of the ectodomain are noted (clip in yellow, core in orange, flap in red), as are the antigenic regions as they are known to exist on the RABV G: site I (residues 224–229), site II (34–42 and 198–200), site III (330–338), site IV (263–264), and minor site “a” (342–343).

R333E, attenuating mutation at RABV G residue 333; TM, transmembrane domain. See also Figures S2 and S3.