Table 2.
Glycopeptides of A1AT with Glu-C/Chymotrypsin Digestiona
| peptide | glycan | experimental m/z | charge (z) | theoretical m/z | Δppm | Y1 ion |
|---|---|---|---|---|---|---|
| with glycosylation site Asn70 | ||||||
| AHQSnSTNIF | A2G2S2(54200)* | 1108.4446 | 3+ | 1108.4363 | 7.5 | 1321.6060 |
| FA2G2S2(54201)* | 1157.1300 | 3+ | 1157.1233 | 6.7 | ||
| AHQSnSTNIFF | A2G2S2(54200) | 1157.4679 | 3+ | 1157.4589 | 7.8 | |
| FA2G2S2(54201) | 1206.1545 | 3+ | 1206.1449 | 8.0 | ||
| with glycosylation site Asn107 | ||||||
| nLTEIPE | A2G2S2(54200)* | 1007.4070 | 3+ | 1007.4003 | 6.7 | 1018.4965 |
| FA2G2S2(54201)* | 1056.0955 | 3+ | 1056.0863 | 8.7 | ||
| A3G3S3(65300)* | 1226.1520 | 3+ | 1226.1428 | 7.5 | ||
| A3FG3S3(65301)* | 1274.8389 | 3+ | 1274.8288 | 7.9 | ||
| with glycosylation site Asn271 | ||||||
| LGnATAIF | A2G2S2(54200)* | 1004.4170 | 3+ | 1004.4090 | 8.0 | 1009.5201 |
| A2G2S2(54200)* | 1506.1220 | 2+ | 1506.1135 | 5.6 | ||
| GnATAIF | A2G2S2(54200) | 966.7211 | 3+ | 966.7169 | 4.3 | |
aGlycopeptides selected for PRM quantification are labeled with an asterisk, and corresponding Y1 ions are shown.