KEY RESOURCES TABLE
| REAGENT OR RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-GFP | Invitrogen | Cat#A-11122; RRID: AB_221569 |
| Goat polyclonal anti-Choline Acetyltransferase (ChAT) | Millipore | Cat#AB144P; RRID:AB_2079751 |
| Donkey anti-rabbit Alexa Fluor 488 | Invitrogen | Cat#A-21206; RRID:AB_2535792 |
| Donkey anti-goat Alexa Fluor 594 | Invitrogen | Cat# A-11058; RRID:AB_2534105 |
| Chemicals, Peptides and Recombinant proteins | ||
| Vectashield | Vector Laboratories | Cat# H-1400; RRID: AB_2336787 |
| Neurobiotin | Vector Laboratories | Cat# SP-1120; RRID: AB_2313575 |
| Streptavidin Alexa Fluor 594 conjugate | Invitrogen | Cat# S11227; RRID:AB_2337250 |
| QX 314 Chloride | Tocris | Cat# 2313 |
| Ames’ Media | Sigma | Cat# A1420–10X1L |
| SR 95531 hydrobromide (gabazine) | Tocris | Cat# 1262 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.Cg-Tg(Hlxb9-GFP)1Tmj/J (Hb9:GFP) | The Jackson Laboratory | RRID: IMST_JAX:005029 |
| Software and Algorithms | ||
| Simple Neurite Tracer FIJI plugin | NIH | https://imagej.net/SNT |
| FIJI | NIH | https://imagej.nih.gov/ij; RRID:SCR_003070 |
| MATLAB | Mathworks | https://www.mathworks.com/products/matlab.html;; RRID: SCR_001622 |
| FIJI Dendrite analysis macro | This paper | |
| Scanlmage4 | (Pologruto et al., 2003) | http://scanimage.vidriotechnologies.com/display/SIH/ScanImage+Home; RRID: SCFL014307 |
| Clampex 10.3 | Molecular Devices | RRID: SCR_011323 |