TABLE 2.

Responses of selected transcripts to the import of cystine^a

Name	Fold change	Function	Cysteine content (%)
yhaO	130	Putative cysteine importer	1.1
cysP	−94	Thiosulfate binding protein	0
cysU	−62	Sulfate/thiosulfate importer	0.7
yhaM	56	Putative cysteine desulfidase	1.7
tcyP	−37	Cystine importer	0.2
livJ	−7	BCAA transporter subunit	0.6
livK	−3	Leucine transporter subunit
livH	−4	BCAA transporter subunit
livM	−4	BCAA transporter subunit
livG	−5	BCAA transporter subunit
livF	−5	BCAA transporter subunit
brnQ	−1	BCAA transporter	1.1
tcyJ	−2	Cystine importer binding protein	0
tcyL	−1	Cystine importer subunit
tcyN	−1	Cystine importer subunit
cycA	2	Alanine/serine/glycine/cycloserine transporter	1.3
yaaJ	2	Putative alanine/glycine transporter	1.7

^aSulfate-grown cells were exposed to 50 μM cystine for 10 min, and RNA sequence analysis was performed. The full data set can be found in reference 11. Gray shading indicates genes known to be regulated by the transcriptional activator CysB. The cysteine content of CysB-regulated proteins averages 0.2% compared to 1.7% for the entire genome and 0.8% for amino acid importers.