FIG 6.
SEC recruitment to viral IE genes during initiation of viral reactivation from latency. (A) Data from ChIP assays showing the levels of the SEC (mAff4) associated with viral (ICP4 and UL36) and cellular (mHspa1a) genes in latently infected trigeminal ganglia at 0 h post-explant (latent) and 6 h post-explant (reactivation). Data are means ± SEM of results from 2 experiments; each immunoprecipitation was performed with a pool of 10 ganglia. (B) mRNA levels of mAff4 and mHspa1a in explanted ganglia relative to latently infected ganglia. mRNA levels were normalized based on the levels of cellular mGapdh mRNA. Data represent means of results from 2 pools of 4 ganglia each.