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. 2018 Jul 16;2(3):NS20180141. doi: 10.1042/NS20180141

Figure 7. Transfection with TAF1 variant p.Ser1600Gly significantly alters cell proliferation and cyclin expression at both the gene and protein levels.

Figure 7

(A) SH-SY5Y cells were transfected with either an empty vector control, a TAF1 wild-type plasmid expression vector or TAF1 variant p.Ser1600Gly plasmid expression vector. A cell proliferation assay kit was used for measurement of cell proliferation 48 h after the transfection. The percentage of cell proliferation in each sample was calculated respective to the absorbance of empty vector control samples. (B) SH-SY5Y cells were transfected with either an empty vector control, TAF1 wild-type plasmid expression vector or TAF1 variant p.Ser1600Gly plasmid expression vector. β-Actin (HKG), FOS (MIM: 164810), CCNA2 (MIM: 123835), and CCND1 mRNA expression were analyzed using qRT-PCR. Gene expression of the TAF1 variant p.Ser1600Gly was calculated relative to β-Actin. (C) Protein lysates were subjected to Western blot analysis with antibody directed against Cyclin-D1; β-Actin served as a loading control. Band intensities were quantitated from the 16-bit digital image by densitometry in ImageJ (NCBI) and are shown normalized to β-Actin. All data were representative of three experiments with each sample repeated in triplicate.