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. 2018 Dec 3;2(4):NS20180132. doi: 10.1042/NS20180132

Figure 7. Two-photon auto-fluorescence of dopamine in dopaminergic MN9D cells and in glia.

Figure 7

(A,D) Collection of MN9D cells before (A) and 15 min after (D) treatment with 100 μM of PCA. The signal intensity decreases by 34 ± 5%. Vehicle-treated control cells are shown under identical imaging conditions in (C) (before) and (F) (after). The control cells show 1 ± 3% increase in the same period. (B,E) Glia treated with vehicle (B) and 10 mM dopamine for 4 h (E). Fluorescence intensities are false color coded. For (B,E), the brightness is 3× enhanced. Scale bar = 10 μm. (Reprinted with permission from Sarkar, B.; Banerjee, A.; Das, A.K.; Nag, S.; Kaushalya, S.K.; Tripathy, U.; Shameem, M.; Shukla, S.; Maiti, S. (2014) Label-free dopamine imaging in live rat brain slices. ACS Chem. Neurosci., 5(5), 329–334 [49]). Copyright (2014) American Chemical Society.)