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(A) Analysis of c-di-AMP hydrolysis by rMbovP328 and mutated version of rMbovP328. (B) Analysis of pApA hydrolysis by rMbovP328 and mutated version of rMbovP328. NC, negative control. The assay conditions are described in Materials and Methods. (C) Analysis of c-di-GMP and pGpG hydrolysis by rMbovP328. (D) HPLC analysis of the hydrolysis of c-di-AMP, c-di-GMP, pApA, and pGpG by rMbovP327.
