Fig 5. The Mbov_0328/0327 locus is required for nucleotide production in M. bovis.

(A and B) Nucleotide production is important for M. bovis proliferation under cell co-culture conditions. M. bovis parental strain (HB0801), mutant T9.386 (T9.386), and mutant T9.386 transformed with plasmid pCN-T9.386 (CNT9.386) were co-cultivated with EBL cells. The cell culture medium was supplemented with increasing concentration of NMPs (A) or nucleosides (B), and mycoplasma titers were determined at 72 h. Mycoplasma titers were determined at each 24 h interval. The data are presented as the means of three independent assays. Standard deviations are indicated by error bars.