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. 2020 Jul 17;13:6967–6974. doi: 10.2147/OTT.S236476

Figure 3.

Figure 3

MFI2-AS1 is the sponge for miR-125a-5p. (A) Binding site with miR-125a-5p in MFI2-AS1 and strategy for luciferase reporter construction. WT: wild-type. MUT: mutant. (B and C) miR-125a-5p inhibited the luciferase activity of WT-MFI2-AS1 reporter in TPC-1 and K1 cells. NC: negative control. (D and E) Lysates of TPC-1 and K1 cells were utilized for RIP experiment and RNA enrichment was measured by qRT-PCR. (F) MFI2-AS1 knockdown promoted miR-125a-5p expression. (G) miR-125a-5p was negatively correlated with MFI2-AS1 in thyroid cancer tissues. *p<0.05.