Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Caenorhabditis elegans, hermaphrodite) | Wild type | Caenorhabditis Genetics Center (CGC) | N2 | |
| Strain, strain background (Caenorhabditis elegans, hermaphrodite) | spe-9(hc88); rrf-3(b26) | Caenorhabditis Genetics Center (CGC) | TJ1060 | temperature sensitive-sterile strain |
| Strain, strain background (Caenorhabditis elegans, hermaphrodite) | zIs356 [Pdaf-16::daf-16a/b::gfp + rol-6(su1006)] |
Caenorhabditis Genetics Center (CGC) |
TJ356 | DAF-16 reporter strain |
| Antibody | 4-HNE protein adduct antibody (goat polyclonal) | Millipore | Cat.#: AB5605 | (1:2000 dilution) |
| Antibody | anti-Tubulin antibody (mouse monoclonal) | Sigma-Aldrich | Cat.#: T6074 | (1:10,000 dilution) |
| Commercial assay or kit | Thiobarbituric acid reactive substances (TBARS) assay kit | Caymen Chemical | Cat.#: 10009055 | |
| Commercial assay or kit | BCA Protein assay kit | Pierce | Cat.#: 23225 | |
| Chemical compound, drug | Diethyl maleate | Sigma-Aldrich | DEM Cat.#: D97703-100G |
|
| Chemical compound, drug | Liproxstatin | Marcus Conrad (Helmholtz Zentrum München; initially) and subsequently ApexBio Tech LLC | Lip-1 | N-[(3-chlorophenyl) methyl]-spiro[piperidine-4,2'(1'H)-quinoxalin]−3'-amine) |
| Chemical compound, drug | Salicylaldehyde isonicotinoyl hydrazone | Des Richardson (University of Sydney) | SIH | |
| Software, algorithm | GeoPIXE 7.3, Dynamic Analysis | CSIRO | Ryan, 2000. Quantitative trace element imaging using PIXE and the nuclear microprobe. Int J Imag Syst Tech 11, 219–230. | |
| Software, algorithm | ImageJ WormSizer plugin | Moore, B.T., Jordan, J.M., and Baugh, L.R. (2013). WormSizer: high-throughput analysis of nematode size and shape. PLoS One 8, e57142. |
||
| Software, algorithm | Prism v7.0a, GraphPad Software | www.graphpad.com | ||
| Software, algorithm | R package flexsurv | rdocumentation.org/packages/flexsurv | ||
| Software, algorithm | Image J, wrMTrck plugin | Nussbaum-Krammer, C.I., Neto, M.F., Brielmann, R.M., Pedersen, J.S., and Morimoto, R.I. (2015). J Vis Exp, 52321 |
||
| Other | propidium iodide | Life Technologies | P3566 | 0.25 mg/mL solution |