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. 2020 Jul 15;11:446. doi: 10.3389/fendo.2020.00446

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Copyright © 2020 Ma, Shi, Morshed, Latif and Davies.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differentiation of definitive thyroid endoderm from human iPSCs (LF cells). (A) qPCR analysis of thyroid transcriptional factors NKX2-1 and PAX8 in human ES cells and human iPSCs (LF cells). Fold change is represented as the mean ± SEM of three independent experiments. (B) Immunostaining of NKX2-1 (Red) and PAX8 (Green) in human iPSCs (LF cells) derived thyroid endoderm cells. Scale bar = 20 μm. (C) Representative flow cytometry of NKX2-1 and PAX8 expressing cells in untreated (upper), activin A treated (middle) and activing A + Ethacridine (lower) cells. Data were representative of three separate experiments.