Table 1.
Antibodies used in the described Methods.
| Name | Host | Source | Catalog # | ~Dilution | ||
|---|---|---|---|---|---|---|
| Immuno fluorescence | Flow Cytometry | |||||
| Primary antibodies | Polyclonal to OCT4 antibody | Rabbit | Cell Signaling Technology, Danvers, MA | 2,750 | 1:200 | 1:200 |
| mAb to SOX2 | Rabbit | Cell Signaling Technology, Danvers, MA | 3,579 | 1:400 | 1:300 | |
| mAb to TRA1-81 | Mouse | Cell Signaling Technology, Danvers, MA | 4,745 | 1:1000 | 1:1500 | |
| Polyclonal to NANOG antibody | Rabbit | Cell Signaling Technology, Danvers, MA | 3,580 | 1:800 | 1:400 | |
| Monoclonal to FOXA2 antibody | Mouse | Thermo Fisher Scientific, Waltham, MA | MA5-15,542 | 1:500 | 1:500 | |
| Monoclonal to SOX17 antibody | Rabbit | Cell Signaling Technology, Danvers, MA | 81,778 | 1:1000 | 1:1000 | |
| Monoclonal to NKX2-1 antibody | Rabbit | Abcam, Cambridge, UK | ab76013 | 1:400 | 1:500 | |
| Monoclonal to PAX8 antibody | Mouse | Thermo Fisher Scientific, Waltham, MA | MA1-117 | 1:100 | 1:100 | |
| Monoclonal to TAZ antibody | Rabbit | Cell Signaling Technology, Danvers, MA | 8,418 | 1:100 | – | |
| Monoclonal to TG antibody | Rabbit | Abcam, Cambridge, UK | ab156008 | 1:200 | – | |
| Monoclonal to NIS antibody | Mouse | Thermo Fisher Scientific, Waltham, MA | MA5-12,308 | 1:500 | – | |
| Polyclonal NIS | Rabbit | Self-generated by Genscript, Piscataway, NJ. | – | 0.2 ug IgG/106 cells | ||
| mAb TSHR | Mouse | RSR Ltd, Cardiff, UK | RSR-4 | – | 1 ug IgG/106 cells | |
| Polyclonal secondary antibodies | Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) | Goat | Cell Signaling Technology, Danvers, MA | 4,412 | 1:1000 | 1:1000 |
| Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) | Goat | Cell Signaling Technology, Danvers, MA | 4,408 | 1:1000 | 1:1000 | |
| Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) | Goat | Cell Signaling Technology, Danvers, MA | 4,413 | 1:1000 | 1:1000 | |
| Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) | Goat | Cell Signaling Technology, Danvers, MA | 4,409 | 1:1000 | 1:1000 | |
| Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) | Goat | Cell Signaling Technology, Danvers, MA | 4,414 | 1:1000 | 1:1000 | |