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. 2020 Jul 15;11:762. doi: 10.3389/fgene.2020.00762

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Copyright © 2020 Machado, Macabelli, Collado, Meirelles, Guimarães and Chiaratti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photoirradiated mitochondria injected into zygotes are selected against during early embryogenesis. CMXRos-loaded zygotes containing mtDNA of NZB origin were photoirradiated for either 0 (P0) or 20 (P20) s to induce mitochondrial damage. Mitochondria from these zygotes were injected by cytoplasmic transfer (CT) into B6 zygotes, resulting in CT-P0 and CT-P20 groups, respectively. CT-derived embryos were assessed at zygote and blastocyst stage as for the levels of NZB (A) and total (B) mtDNA. Different letters over bars depict statistical difference within group (P < 0.05). *Statistical difference within stage (P = 0.008).