TABLE 4.
Twenty-four-hour fecal excretion of δTE, γTE, and their metabolites in rats after a single gavage of δTE/γTE in comparison with baseline1
Baseline, nmol | δTE/γTE supplement, nmol | Total excretion,2 % | |
---|---|---|---|
γTE | 243 ± 74 | 1110 ± 75* | 36.3 ± 0.9 |
δTE | 8.92 ± 1.91 | 3430 ± 530* | 17.4 ± 2.9 |
γ-CEHC | 9.22 ± 1.55 | 15.9 ± 3.3* | |
δ-CEHC | 1.63 ± 0.22 | 28.6 ± 6.3* | |
γTE-7′ | 4.92 ± 0.12 | 15.5 ± 1.3 | |
δTE-7′ | n.d.3 | 9.39 ± 0.97* | |
γTE-9′ | 4.89 ± 0.54 | 21.3 ± 3.6* | |
δTE-9′ | n.d.3 | 27.0 ± 3.1* | |
γTE-11′ | 17.7 ± 2.0 | 65.2 ± 9.8* | |
δTE-11′ | 0.81 ± 0.08 | 161 ± 16* | |
γTE-13′ (2DB) | 29.4 ± 4.4 | 116 ± 23* | |
γTE-13′ (3DB) | 6.72 ± 1.52 | 24.8 ± 4.3* | |
δTE-13′ (2DB) | 1.42 ± 0.21 | 452 ± 57* | |
δTE-13′ (3DB) | 0.54 ± 0.21 | 451 ± 67* | |
γTE-13′OH | 6.54 ± 0.89 | 23.9 ± 3.5* | |
δTE-13′OH | 0.66 ± 0.07 | 245 ± 28* | |
γTE-13′S4 | 1.60 ± 0.32 | 18.0 ± 3.3* | |
δTE-13′S4 | n.d.3 | 102 ± 15* | |
Total δ-metabolites | 5.14 ± 0.80 | 1480 ± 192* | 7.49 ± 0.98 |
Total γ-metabolites | 80.9 ± 11.4 | 301 ± 52* | 9.21 ± 1.32 |
Values are mean ± SEM, n = 6. Student's t test was used for comparing δTE/γTE supplement with baseline. *P < 0.05, difference between baseline and δTE/γTE supplement. Abbreviations of vitamin E forms and metabolites are shown in Figure 1. CEHC, 2-(β-carboxyethyl)-6-hydroxychroman, also called 3′-COOH; COOH, carboxychromanol; DB, double bonds on the side chain of carboxychromanol; n.d., nondetectable; 13′-OH, 13′-hydroxychromanol; 13′S, sulfated 13′-COOH; γTE, γ-tocotrienol; δTE, δ-tocotrienol.
Total excretion % = (24-h fecal excretion in response to δTE/γTE minus 24-h baseline excretion)/intake.
Detection limit: 0.04–0.2 pmol on column.
γTE-13′S and δTE-13′S were presented as the sum of 2DB and 3DB.