Fig. 2.

Genomic profiling between primary tumors (P) and matched metastases (M1). (A) Frequencies of deleterious non-synonymous variants between primary and metastatic tumors compared with the Cancer Genome Atlas (TCGA) data. (B) The schematic concept of concordant, primary-specific, and metastasis-specific variants and schematic illustration of mutational landscape. (C) Comparison of the percentage of mutations identified as primary-specific, metastasis-specific, or concordant between primary and matched metastases. Twelve patients who did not have deleterious non-synonymous variants were excluded from the comparison. The distribution of number of types of variants per patient is shown at the bottom. (D) Frequencies based on the different protein changes of key genes and comparison with TCGA data. X, variant count (number of variants); Y, each position. Different protein changes represent different variant positions. TP53 shows only the top 30 variant positions. The highest frequencies of the top three variant’s positions compared to the TCGA data are shown at the bottom of each graph. The data were calculated based on 158 primary tissue samples. TCGA data were obtained from 212 tissue samples.