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. 2020 Aug;26(8):1069–1078. doi: 10.1261/rna.075945.120

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© 2020 Prezza et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Initial DASH run on Salmonella total RNA. (A) Composition of total RNA extracted from exponentially growing Salmonella as deduced from RNA-seq reads. (B) Conservation of Salmonella rRNAs genes and location of the identified sgRNA target sites. The per-base conservation of each rRNA gene across all Salmonella rRNA homologs is plotted. Above each consensus sequence (represented as a gray rectangle), the designed sgRNAs target sites are indicated by a black triangle. (C) Pilot run of DASH-mediated rRNA depletion in Salmonella. (Left) The fraction of ribosomal-derived reads over the total number of mapped reads for a control and a DASH-treated library. (Right) Same data are expressed as efficiency of DASH-mediated rRNA depletion.