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. 2020 Aug;26(8):1069–1078. doi: 10.1261/rna.075945.120

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© 2020 Prezza et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 3. — Optimization of DASH reaction conditions. (A) rRNA depletion efficiency upon DASH cleavage with varying amounts of Cas9 and the sgRNA pool. Cas9 and sgRNA amounts are indicated as molar excess over a single fragment of the cDNA library. (B) Impact of preincubation of sgRNA and Cas9 prior to DASH on rRNA depletion efficiency. (C) Length distribution of the mapped portions of reads aligning to rRNAs after DASH compared with an untreated control. Frequency values are expressed as fraction (%) of the total number of reads mapping to rRNAs. (D) Same as in C but for reads aligning to non-rRNA genes.