FIGURE 5.

DASH of low-input RNA samples. (A) DASH efficiency for steadily decreasing input RNA amounts and for different Cas9 removal methods. DASH was performed on 1/5th of the cDNA resulting from reverse transcription. Cas9 and the sgRNA pool were used in a 1000 and 10,000 excess over a single fragment of the cDNA library, respectively. The indicated RNA amounts correspond to the reverse transcription input, while the cDNA amounts refer to what was used for DASH. (B) Depletion efficiency of DASH (3500:35,000 excess of Cas9:sgRNA) when combined with different library preparation kits. The indicated RNA amounts correspond to the reverse transcription input. (C) RNA class distribution of sequencing reads in the control and DASH samples shown in panel B.