FIG 1.

Individual ColIb+ Salmonella enterica serovar Typhimurium (S. Tm) lyse by temperate phage-mediated cell lysis in response to SOS stress. (A) The schematic diagram shows that ColIb release in S. Tm is mediated by λ-like prophages. (B) Mean fluorescence intensity (MFI) levels of S. Tm^WT p^{Pcib gfp} and S. Tm^Ph p^{Pcib gfp} and their respective negative controls S. Tm^WT and S. Tm^Ph were determined by flow cytometry. Bacteria were grown in LB media containing respective supplements (0.5 μg/ml MitC; 100 μM DTPA) for 4 h. The OD₆₀₀ was monitored hourly, and samples were taken for flow cytometric analysis. Data from three independent experiments are shown. Statistical differences determined using two-way ANOVA with Bonferroni’s posttest (***, P < 0.001). Bars represent means and standard deviations (SD) (error bars).