FIG 2.

The primary screen identifies compounds that prevent the yeast strain (Sc<CnMPR1>) from crossing the BBB. (A) A phytochemical-derived natural product library (n = 240) was screened 3 times. The fluorescence readout from the bottom well was averaged and ranked based on statistical significance. Of the 240 compounds tested, 34 compounds resulted in the greatest reduction in fluorescence (P ≤ 0.001, dark blue squares), 108 compounds had no effect (gray squares), and the remaining 84 compounds had variable and modest effects compared to the DMSO control.