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. 2020 Jul 21;11(4):e01249-20. doi: 10.1128/mBio.01249-20

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Copyright © 2020 Aaron et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Proteolytic activity of Mpr1 protein is inhibited by the lead compounds. Increasing concentrations of the three lead compounds inhibit the activity of recombinant Mpr1 protein, and the inhibition is statistically significant compared to DMSO control (A to C). In contrast, a compound that failed the primary screen did not inhibit the proteolytic activity of Mpr1 (D). Recombinant CnMpr1-His-tagged protein was isolated via nickel-NTA magnetic beads from a culture of Pichia pastoris transformed with a plasmid expressing CnMPR1-HIS cDNA as previously described (47). A FITC-labeled casein assay was used to assess the proteolytic activity of Mpr1. The Mpr1 activity in solvent (DMSO) is shown as a positive control for the proteolytic assay (blue circles). **, P < 0.01, ***, P < 0.001.