Figure 2: Effects of 10% cyclic mechanical stretch on cell size, viability and release of EVs from MLE-12 cells.

MLE-12 cells grown in culture media containing EV-depleted FBS were exposed to 10% cyclic stretch for 24 hours. (A) Bright field microscopic images in the upper captured at x 20 magnification and corresponding binary images of the digitally isolated cells are shown in the lower panels. Area and perimeter were used as determinants for the cell-size and are shown in the bar diagrams. (B) Cells from control and stretched experiments were recovered from the plates by trypsinization and counted for live cells number using cellometer. (C) EVs were isolated from the culture media after experimental condition; the concentration of EVs was evaluated by NanoSight. Data were normalized to the number of live cells recovered from the plate after experiments. Results represent the average of three independent experiments ± SEM, each done at least in duplicate. *p<0.05 and ****p <0.0001