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. 2020 Jul 21;10:12057. doi: 10.1038/s41598-020-68970-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Laser confocal microphotographs of hippocampal cultures from control (A), and tbl (B) mice illustrative of MPA2 (green) and CLT (red) immunocytochemistry. (C–F), graphical representation of the fluorescence intensities of MAP2 and CLT in absolute values per image (C,E); and relative to the % of measured area (D,F). In both absolute (C,E) and relative (D,F), values, MAP2 and CLT expressions are higher in control cultures than in tbl ones. The asterisks indicate significant differences in the Student’s t test: ***p = 0.0008 (C); **p = 0.005 (D); ***p = 0.0005 (E); and ***p = 4.4^E−06 (F). Bars = 50 µm. a.u., arbitrary units.