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. 2020 Jul 21;10:12057. doi: 10.1038/s41598-020-68970-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ImageJ composites illustrative of the expression of HERC1 (red) and EEA1 (green), in control (A) and tbl (B) hippocampal cultures. (C–E), graphs show the intensity of the expression of HERC1 (C) and EEA1 (D). HERC1immunoreactivity is higher in tbl relative to control cultures (C, *p = 0.0404). Likewise, EEA1 expression is lower in control cultures relative to tbl ones (D, *p = 0.04276). Bars = 5 µm. n, neuronal cell nucleus.