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. 2020 Jul 21;11(7):553. doi: 10.1038/s41419-020-02747-9

Fig. 2. CircTADA2A inhibited growth-factors-driven fibroblasts proliferation and activation.

Fig. 2

LL-24 cells were transfected with a si-circTADA2A or its negative control (si-control) or b adenovirus vector (Ad)-circTADA2A or its negative control (Ad-GFP). Cell proliferation was measured by BrdU incorporation assay after stimulation of 5% FCS or 60 ng/ml PDGF-BB or 200 ng/ml IGF-1 for 6 h. The protein expression of collagen 1a1 (COL1A1), collagen 3a1 (COL3A1), laminin (LN), fibronectin (FN), and α-smooth muscle actin (α-SMA) were determined by western blot after stimulation of 10 ng/ml TGF-β1 for 6 h, GAPDH was used as an internal control. *P < 0.05, **P < 0.01.