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. 2020 Jul 21;11:3659. doi: 10.1038/s41467-020-17475-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The regulatory structure of the co-repressive consortium in which each strain downregulates production of QS molecule and fluorescent protein in the other strain. b A detailed genetic circuit diagram of the co-repressive consortium. The cyan strain produces C4-HSL via the RhlI QS synthase. C4-HSL induces the expression of LacI-11 in the yellow strain. LacI-11 shuts off CinI and sfYFP in the yellow strain. 3OHC14-HSL produced by the CinI QS synthase in the yellow strain induces expression of RbsR-L in the cyan strain. RbsR-L shuts off RhlI and sfCFP in the cyan strain.