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Initial rates of CENTA hydrolysis by NDM-1 vs. PDTC concentration with (a) 10 μM ZnSO4, (b) 50 μM ZnSO4 or (c) 100 μM ZnSO4 supplementing the reaction buffer. In each case, NDM-1 activity was reduced as the PDTC concentration exceeded two equivalents of the ZnSO4 in the reaction, denoted by bold type on the x-axis.
