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. 2020 Jul 22;10:12518. doi: 10.1038/s41598-020-69593-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CG4 cells display an OPCs-like morphology in the undifferentiated state (A) and oligodendrocyte-like phenotype in the differentiated state (B). PV (Pvalb) transcript levels were determined by semi-quantitative PCR after 30 cycles (upper) and by RT-qPCR (lower), where Cypa was used as reference gene (C,D). PV expression levels were determined by Western blot analyses, where GAPDH signals were used as loading control (E,F). The morphological features of LV-infected CG4 cells (GFP, PV, shPV) appeared similar to the control (C) in both undifferentiated OPC-like and differentiated OLG-like states (G,H). Images A, B, G and H were acquired with the IncuCyte system. The same raw data (movie) files were also used to select representative cells shown in Figs. 6A, B and 7A, B. All movie files are available in the ZENODO repository https://doi.org/10.5281/zenodo.3907289¹. The individual frames from which the representative cells were taken are marked and annotated in the “Supporting Information” (.pdf) file available in the same repository.